Facts about Polymerase Chain Reaction "PCR"

The Polymerase Chain reaction "PCR" is a laboratory technique for generating large quantities of specified DNA.In other words, it is a cell free amplification technique used to generate large quantities of specified DNA. This post presents some basic facts that you cannot miss about PCR.
1. The technique of PCR was developed by Karry Mullis is 1984 by Karry Mullis who got Nobel Prize in 1993.

2. PCR acts as a photocopy machine in in a molecular biology laboratory

Principle of PCR

3. The process consists mainly of three steps viz. Denaturation, Renaturation and Synthesis...these processes are repeated again and againto geberate multiple forns of target DNA.

4. PCR is modified as per the specific demand of the situation. Different types of PCR used are:
  • Nested PCR
  • Inverse PCR
  • Anchored PCR
  • Reverse transcription PCR
  • Asymmetric PCR
  • Real-time quantitative PCR
  • RAPD (Random amplified polymorphic DNA)
  • Real time quantitative PCR
  • Amplified fragment length polymorpjism 
  • Rapid amplification of cDNA ends 
5. PCR has wide variety of uses which shall be discussed later 


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