Procedures of gene cloning

A clone refers to a group of organisms, cells or molecules arising from a single individual.The general procedures in gene cloning are:

1. Generation of desired DNA fragments:

via the methods of

insertion of  fragments into a cloning vector

via :
  • Ligation of blunt or cohesive ends 
  • Homopolymer tailing 
  • linker molecules 

Introduction into host cells

  • Transformation 
  • transduction 
  • transfection 

Selection or screening

  • Hybridisation 
  • PCR 
  • immunochemical methods 
  • protein-protein interactions 
  • functionl complementation 

Flowchart of gene cloning procedure

gene cloning, gene cloning procedures


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